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Hv LUX 1 is a candidate gene underlying the early maturity 10 locus in barley: phylogeny, diversity, and interactions with the circadian clock and photoperiodic pathways
Author(s) -
Campoli Chiara,
Pankin Artem,
Drosse Benedikt,
Casao Cristina M.,
Davis Seth J.,
Korff Maria
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12346
Subject(s) - biology , circadian clock , hordeum vulgare , photoperiodism , circadian rhythm , eudicots , hordeum , locus (genetics) , gene , genetics , arabidopsis , phylogenetic tree , evolutionary biology , botany , poaceae , neuroscience , mutant , taxonomy (biology)
Summary Photoperiodic flowering is a major factor determining crop performance and is controlled by interactions between environmental signals and the circadian clock. We proposed Hvlux1 , an ortholog of the Arabidopsis circadian gene LUX ARRHYTHMO , as a candidate underlying the early maturity 10 ( eam10 ) locus in barley ( Hordeum vulgare L.). The link between eam10 and Hvlux1 was discovered using high‐throughput sequencing of enriched libraries and segregation analysis. We conducted functional, phylogenetic, and diversity studies of eam10 and Hv LUX 1 to understand the genetic control of photoperiod response in barley and to characterize the evolution of LUX ‐like genes within barley and across monocots and eudicots. We demonstrate that eam10 causes circadian defects and interacts with the photoperiod response gene Ppd‐H1 to accelerate flowering under long and short days. The results of phylogenetic and diversity analyses indicate that Hv LUX 1 was under purifying selection, duplicated at the base of the grass clade, and diverged independently of LUX ‐like genes in other plant lineages. Taken together, these findings contribute to improved understanding of the barley circadian clock, its interaction with the photoperiod pathway, and evolution of circadian systems in barley and across monocots and eudicots.