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The mechanism of ORFH 79 suppression with the artificial restorer fertility gene Mt‐ GRP 162
Author(s) -
Hu Jun,
Huang Wenchao,
Huang Qi,
Qin Xiaojian,
Dan Zhiwu,
Yao Guoxin,
Zhu Renshan,
Zhu Yingguo
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12310
Subject(s) - cytoplasmic male sterility , biology , sterility , gene , transgene , gene silencing , microbiology and biotechnology , gene expression , mitochondrion , genetics , oryza sativa
Summary The restoration fertility complex ( RFC ) was previously identified in Honglian ( HL )‐cytoplasmic male sterility ( CMS ) rice ( Oryza sativa ), and glycine‐rich protein 162 ( GRP 162) is responsible for binding to the CMS ‐associated transcript atp6‐orfH79 . Here, we engineered a recombinant GRP 162 containing the mitochondrial transit peptide, termed Mt‐ GRP 162, as an artificial restorer of fertility ( Rf ) gene. Mt‐ GRP 162 was confirmed to bind to CMS ‐associated RNA and to localize to the mitochondria. The transgenic plants showed restored fertility with partially functional pollen. We found that the expression of ORFH 79 decreased in transgenic plants, while the expression of atp6‐orfH79 was not changed. These findings indicate that Mt‐ GRP 162 restores fertility by suppressing the expression of the cytotoxic protein ORFH 79 at the post‐transcriptional level rather than via the cleavage of atp6‐orfH79 in the presence of RFC . These findings contribute to our understanding of the mechanisms of restoration through diverse pathways.