Premium
Identification of a novel micro RNA (mi RNA ) from rice that targets an alternatively spliced transcript of the N ramp6 ( N atural resistance‐associated macrophage protein 6 ) gene involved in pathogen resistance
Author(s) -
Campo Sonia,
PerisPeris Cristina,
Siré Christelle,
Moreno Ana Beatriz,
Donaire Livia,
Zytnicki Matthias,
Notredame Cedric,
Llave César,
San Segundo Blanca
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12292
Subject(s) - rna , biology , rna silencing , microrna , riboswitch , gene expression , gene , plant disease resistance , elicitor , transcriptome , microarray analysis techniques , rna binding protein , rna extraction , rna editing , rna interference , microbiology and biotechnology , non coding rna , genetics
Summary Plants have evolved efficient defence mechanisms to defend themselves from pathogen attack. Although many studies have focused on the transcriptional regulation of defence responses, less is known about the involvement of micro RNA s (mi RNA s) as post‐transcriptional regulators of gene expression in plant immunity. This work investigates miRNAs that are regulated by elicitors from the blast fungus M agnaporthe oryzae in rice ( O ryza sativa ). Small RNA libraries were constructed from rice tissues and subjected to high‐throughput sequencing for the identification of elicitor‐responsive mi RNA s. Target gene expression was examined by microarray analysis. Transgenic lines were used for the analysis of mi RNA functioning in disease resistance. Elicitor treatment is accompanied by dynamic alterations in the expression of a significant number of mi RNA s, including new members of annotated mi RNA s. Novel mi RNA s from rice are proposed. We report a new rice mi RNA , osa‐mi R 7695, which negatively regulates an alternatively spliced transcript of O s N ramp6 ( N atural resistance‐associated macrophage protein 6 ). This novel miRNA experienced natural and domestication selection events during evolution, and its overexpression in rice confers pathogen resistance. This study highlights an mi RNA ‐mediated regulation of O s N ramp6 in disease resistance, whilst illustrating the existence of a novel regulatory network that integrates mi RNA function and m RNA processing in plant immunity.