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Ectopic expression of K ip‐related proteins restrains root‐knot nematode‐feeding site expansion
Author(s) -
Vieira Paulo,
Escudero Carmen,
Rodiuc Natalia,
Boruc Joanna,
Russinova Eugenia,
Glab Nathalie,
Mota Manuel,
Veylder Lieven,
Abad Pierre,
Engler Gilbert,
Almeida Engler Janice
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12255
Subject(s) - meloidogyne incognita , microbiology and biotechnology , biology , root knot nematode , cyclin dependent kinase , ectopic expression , chemistry , cell cycle , nematode , cell , biochemistry , gene , ecology
Summary The development of nematode feeding sites induced by root‐knot nematodes involves the synchronized activation of cell cycle processes such as acytokinetic mitoses and DNA amplification. A number of key cell cycle genes are reported to be critical for nematode feeding site development. However, it remains unknown whether plant cyclin‐dependent kinase (CDK) inhibitors such as the A rabidopsis interactor/inhibitor of CDK ( ICK )/Kip‐related protein ( KRP ) family are involved in nematode feeding site development. This study demonstrates the involvement of A rabidopsis ICK 2/ KRP 2 and ICK 1/ KRP 1 in the control of mitosis to endoreduplication in galls induced by the root‐knot nematode M eloidogyne incognita . Using ICK / KRP promoter‐ GUS fusions and m RNA in situ hybridizations, we showed that ICK 2/ KRP 2 , ICK 3/ KRP 5 and ICK 4/ KRP 6 are expressed in galls after nematode infection. Loss‐of‐function mutants have minor effects on gall development and nematode reproduction. Conversely, overexpression of both ICK 1/ KRP 1 and ICK 2/ KRP 2 impaired mitosis in giant cells and blocked neighboring cell proliferation, resulting in a drastic reduction of gall size. Studying the dynamics of protein expression demonstrated that protein levels of ICK 2/ KRP 2 are tightly regulated during giant cell development and reliant on the presence of the nematode. This work demonstrates that impeding cell cycle progression by means of ICK 1/ KRP 1 and ICK 2/ KRP 2 overexpression severely restricts gall development, leading to a marked limitation of root‐knot nematode development and reduced numbers of offspring.