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The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL ‐related homeobox 3 A ( O s WOX 3 A ) and function in leaf, spikelet, tiller and lateral root development
Author(s) -
Cho SungHwan,
Yoo SooCheul,
Zhang Haitao,
Pandeya Devendra,
Koh HeeJong,
Hwang JiYoung,
Kim GyungTae,
Paek NamChon
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12231
Subject(s) - mutant , biology , auxin , homeobox , morphogenesis , phenotype , axillary bud , gene , microbiology and biotechnology , oryza sativa , botany , gene expression , genetics , explant culture , in vitro
Summary In order to understand the molecular genetic mechanisms of rice ( O ryza sativa ) organ development, we studied the narrow leaf2 narrow leaf3 ( nal2 nal3 ; hereafter nal2/3 ) double mutant, which produces narrow‐curly leaves, more tillers, fewer lateral roots, opened spikelets and narrow‐thin grains. We found that narrow‐curly leaves resulted mainly from reduced lateral‐axis outgrowth with fewer longitudinal veins and more, larger bulliform cells. Opened spikelets, possibly caused by marginal deformity in the lemma, gave rise to narrow‐thin grains. Map‐based cloning revealed that NAL 2 and NAL 3 are paralogs that encode an identical O s WOX 3 A ( O s NS ) transcriptional activator, homologous to NARROW SHEATH 1 ( NS 1) and NS 2 in maize and PRESSED FLOWER in A rabidopsis. O s WOX 3 A is expressed in the vascular tissues of various organs, where nal2/3 mutant phenotypes were displayed. Expression levels of several leaf development‐associated genes were altered in nal2/3 , and auxin transport‐related genes were significantly changed, leading to pin mutant‐like phenotypes such as more tillers and fewer lateral roots. O s WOX 3 A is involved in organ development in rice, lateral‐axis outgrowth and vascular patterning in leaves, lemma and palea morphogenesis in spikelets, and development of tillers and lateral roots.