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Complementary and dose‐dependent action of A t CCS 52 A isoforms in endoreduplication and plant size control
Author(s) -
Baloban Mikhail,
Vanstraelen Marleen,
Tarayre Sylvie,
Reuzeau Christophe,
Cultrone Antonietta,
Mergaert Peter,
Kondorosi Eva
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12216
Subject(s) - endoreduplication , biology , microbiology and biotechnology , ectopic expression , mitosis , polyploid , gene isoform , transgene , ploidy , cell , cell culture , cell cycle , genetics , gene
Summary The dimension of organs depends on the number and the size of their component cells. Formation of polyploid cells by endoreduplication cycles is predominantly associated with increases in the cell size and implicated in organ growth. In plants, the CCS 52 A proteins play a major role in the switch from mitotic to endoreduplication cycles controlling thus the number of mitotic cells and the endoreduplication events in the differentiating cells. A rabidopsis has two CCS 52 A isoforms; A t CCS 52 A 1 and A t CCS 52 A 2. Here we focused on their roles in endoreduplication and cell size control during plant development. We demonstrate their complementary and dose‐dependent actions that are dependent on their expression patterns. Moreover, the impact of CCS 52 A overexpression on organ size in transgenic plants was dependent on the expression level; while enhanced expression of the CCS 52 A genes positively correlated with the ploidy levels, organ sizes were negatively affected by strong overexpression whereas milder overexpression resulted in a significant increase in the organ sizes. Taken together, these finding support both complementary and dose‐dependent actions for the A rabidopsis CCS 52 A isoforms in plant development and demonstrate that elevated ectopic CCS 52 A expression positively correlates with organ size, opening a route to higher biomass production.