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ALFIN ‐ LIKE 6 is involved in root hair elongation during phosphate deficiency in Arabidopsis
Author(s) -
Chandrika Nulu Naga Prafulla,
Sundaravelpandian Kalaipandian,
Yu SuMay,
Schmidt Wolfgang
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12194
Subject(s) - root hair , arabidopsis , mutant , elongation , biology , microbiology and biotechnology , arabidopsis thaliana , gene , meristem , pi , wild type , biochemistry , materials science , ultimate tensile strength , metallurgy
Summary Phosphate (Pi) starvation in plants induces dense and elongated root hairs, which increase the absorptive surface area of the roots and play a critical role in Pi uptake. The molecular mechanism underlying these changes remains unclear. Forward and reverse genetic approaches were employed to identify novel genes involved in root hair formation on Pi starvation. The mutant per2 , with defects in root hair elongation specifically under low Pi conditions, was identified in a large‐scale genetic screen of T‐ DNA insertion lines. The phenotype was caused by a mutation in the homeodomain protein ALFIN ‐ LIKE 6 ( AL 6). From a screen of mutants defective in genes that showed lower transcript abundance in per2 relative to wild‐type roots on low Pi medium, we identified four putative downstream targets of AL 6, namely ETC 1, NPC 4, SQD 2 and PS 2, all of which were critical in root hair elongation of Pi‐deficient plants. The results further indicate that AL 6 is involved in the control of growth and several key responses to Pi starvation. Our findings demonstrate that AL 6 controls the transcription of a suite of genes critical for root hair elongation under low Pi conditions, suggesting a novel physiological function for an Alfin gene in Arabidopsis.