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NITRILASE 1 regulates the exit from proliferation, genome stability and plant development
Author(s) -
Doskočilová Anna,
Kohoutová Lucie,
Volc Jindřich,
Kourová Hana,
Benada Oldřich,
Chumová Jana,
Plíhal Ondřej,
Petrovská Beáta,
Halada Petr,
Bögre László,
Binarová Pavla
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12185
Subject(s) - cytokinesis , microbiology and biotechnology , biology , cell division , cell growth , cell cycle , arabidopsis , gene silencing , cytoplasm , septin , cell , genetics , gene , mutant
Summary Nitrilases are highly conserved proteins with catabolic activity but much less understood functions in cell division and apoptosis. To elucidate the biological functions of A rabidopsis NITRILASE 1, we characterized its molecular forms, cellular localization and involvement in cell proliferation and plant development. We performed biochemical and mass spectrometry analyses of NITRILASE 1 complexes, electron microscopy of nitrilase polymers, imaging of developmental and cellular distribution, silencing and overexpression of nitrilases to study their functions. We found that NITRILASE 1 has an intrinsic ability to form filaments. GFP ‐ NITRILASE 1 was abundant in proliferating cells, distributed in cytoplasm, in the perinuclear area and associated with microtubules. As cells exited proliferation and entered differentiation, GFP ‐ NITRILASE 1 became predominantly nuclear. Nitrilase silencing dose‐dependently compromised plant growth, led to loss of tissue organization and sustained proliferation. Cytokinesis was frequently aborted, leading to enlarged polyploid cells. In reverse, independently transformed cell lines overexpressing GFP ‐ NITRILASE 1 showed slow growth and increased rate of programmed cell death. Altogether, our data suggest that NITRILASE 1 homologues regulate the exit from cell cycle and entry into differentiation and simultaneously are required for cytokinesis. These functions are essential to maintain normal ploidy, genome stability and tissue organization.