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Bruno‐like proteins modulate flowering time via 3′ UTR‐dependent decay of SOC1 mRNA
Author(s) -
Kim HyungSae,
Abbasi Nazia,
Choi SangBong
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12181
Subject(s) - biology , untranslated region , messenger rna , rna binding protein , rna , three prime untranslated region , five prime untranslated region , gene , arabidopsis , mutant , microbiology and biotechnology , leafy , translation (biology) , gene expression , genetics , botany
Summary The Bruno RNA‐binding protein (RBP) has been shown to initially repress the translation of oskar mRNA during Drosophila oogenesis and later to be involved in a broad range of RNA regulation. Here, we show that homologous constitutive overexpression of each of two Arabidopsis thaliana Bruno ‐like genes, AtBRN1 and AtBRN2 , delayed the flowering time, while the atbrn1 atbrn2‐3 double mutant flowered early and exhibited increased expression of APETALA1 ( AP1 ) and LEAFY ( LFY ) transcripts. Crossing of 35S::AtBRN s with SOC 1 101‐D plants demonstrated that 35S::AtBRN s suppress an early‐flowering phenotype of SOC 1 101‐D in which the coding sequence ( CDS ) with the 3′ UTR of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 ( SOC 1 ) gene is overexpressed. However, this early‐flowering phenotype by SOC 1 overexpression was maintained in the plants coexpressing 35S::AtBRN s and 35S:: SOC 1 without the 3′ UTR ( – 3′ UTR ). Using yeast three‐hybrid, electrophoretic mobility shift, RNA immunoprecipitation, and protoplast transient assays, we found that AtBRNs bind to the 3′ UTR of SOC1 RNA and participate in mRNA decay, which was mediated by the distal region of the SOC1 3′ UTR. Overall, AtBRNs repress SOC1 activity in a 3′ UTR‐dependent manner, thereby controlling the flowering time in Arabidopsis.