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UDP ‐glucosyltransferase UGT 84A2/ BRT 1 is required for Arabidopsis nonhost resistance to the Asian soybean rust pathogen Phakopsora pachyrhizi
Author(s) -
Langenbach Caspar,
Campe Ruth,
Schaffrath Ulrich,
Goellner Katharina,
Conrath Uwe
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12155
Subject(s) - phakopsora pachyrhizi , arabidopsis , biology , mutant , genetics , gene , microbiology and biotechnology , botany , fungicide
Summary Nonhost resistance ( NHR ) of plants to fungal pathogens comprises different defense layers. Epidermal penetration resistance of Arabidopsis to Phakopsora pachyrhizi requires functional PEN 1 , PEN 2 and PEN 3 genes, whereas post‐invasion resistance in the mesophyll depends on the combined functionality of PEN 2 , PAD 4 and SAG 101 . Other genetic components of Arabidopsis post‐invasion mesophyll resistance remain elusive. We performed comparative transcriptional profiling of wild‐type, pen2 and pen2 pad4 sag101 mutants after inoculation with P. pachyrhizi to identify a novel trait for mesophyll NHR . Quantitative reverse transcription‐polymerase chain reaction (RT‐qPCR) analysis and microscopic analysis confirmed the essential role of the candidate gene in mesophyll NHR . UDP ‐glucosyltransferase UGT 84A2/bright trichomes 1 ( BRT 1) is a novel component of Arabidopsis mesophyll NHR to P. pachyrhizi . BRT 1 is a putative cytoplasmic enzyme in phenylpropanoid metabolism. BRT 1 is specifically induced in pen2 with post‐invasion resistance to P. pachyrhizi . Silencing or mutation of BRT 1 increased haustoria formation in pen2 mesophyll. Yet, the brt1 mutation did not affect NHR to P. pachyrhizi in wild‐type plants. We assign a novel function to BRT 1, which is important for post‐invasion NHR of Arabidopsis to P. pachyrhizi . BRT1 might serve to confer durable resistance against P. pachyrhizi to soybean.