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Hydrogen peroxide‐regulated genes in the Medicago truncatula – Sinorhizobium meliloti symbiosis
Author(s) -
Andrio Emilie,
Marino Daniel,
Marmeys Anthony,
Segonzac Marion Dunoyer,
Damiani Isabelle,
Genre Andrea,
Huguet Stéphanie,
Frendo Pierre,
Puppo Alain,
Pauly Nicolas
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12120
Subject(s) - medicago truncatula , sinorhizobium meliloti , symbiosis , biology , gene , downregulation and upregulation , microbiology and biotechnology , reactive oxygen species , gene expression , transcriptome , hydrogen peroxide , medicago , root nodule , biochemistry , genetics , bacteria
SummaryReactive oxygen species ( ROS ), particularly hydrogen peroxide (H 2 O 2 ), play an important role in signalling in various cellular processes. The involvement of H 2 O 2 in the Medicago truncatula – Sinorhizobium meliloti symbiotic interaction raises questions about its effect on gene expression. A transcriptome analysis was performed on inoculated roots of M. truncatula in which ROS production was inhibited with diphenylene iodonium ( DPI ). In total, 301 genes potentially regulated by ROS content were identified 2 d after inoculation. These genes included MtSpk1 , which encodes a putative protein kinase and is induced by exogenous H 2 O 2 treatment.MtSpk1 gene expression was also induced by nodulation factor treatment. MtSpk1 transcription was observed in infected root hair cells, nodule primordia and the infection zone of mature nodules. Analysis with a fluorescent protein probe specific for H 2 O 2 showed that MtSpk1 expression and H 2 O 2 were similarly distributed in the nodule infection zone. Finally, the establishment of symbiosis was impaired by MtSpk1 downregulation with an artificial micro‐ RNA . Several genes regulated by H 2 O 2 during the establishment of rhizobial symbiosis were identified. The involvement of MtSpk1 in the establishment of the symbiosis is proposed.