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The B rassica napus blackleg resistance gene LepR3 encodes a receptor‐like protein triggered by the L eptosphaeria maculans effector AVRLM 1
Author(s) -
Larkan N. J.,
Lydiate D. J.,
Parkin I. A. P.,
Nelson M. N.,
Epp D. J.,
Cowling W. A.,
Rimmer S. R.,
Borhan M. H.
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12043
Subject(s) - leptosphaeria maculans , biology , gene , r gene , genetics , blackleg , locus (genetics) , plant disease resistance , brassica , botany
SummaryLepR3 , found in the B rassica napus cv ‘ S urpass 400’, provides race‐specific resistance to the fungal pathogen L eptosphaeria maculans , which was overcome after great devastation in A ustralia in 2004. We investigated the LepR3 locus to identify the genetic basis of this resistance interaction. We employed a map‐based cloning strategy, exploiting collinearity with the A rabidopsis thaliana and B rassica rapa genomes to enrich the map and locate a candidate gene. We also investigated the interaction of LepR3 with the L . maculans avirulence gene AvrLm1 using transgenics. LepR3 was found to encode a receptor‐like protein ( RLP ). We also demonstrated that avirulence towards LepR3 is conferred by AvrLm1 , which is responsible for both the Rlm1 and LepR3 ‐dependent resistance responses in B . napus . LepR3 is the first functional B . napus disease resistance gene to be cloned. AvrLm1 's interaction with two independent resistance loci, Rlm1 and LepR3 , highlights the need to consider redundant phenotypes in ‘gene‐for‐gene’ interactions and offers an explanation as to why LepR3 was overcome so rapidly in parts of A ustralia.