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The tomato S l SHINE 3 transcription factor regulates fruit cuticle formation and epidermal patterning
Author(s) -
Shi Jian Xin,
Adato Avital,
Alkan Noam,
He Yonghua,
Lashbrooke Justin,
Matas Antonio J.,
Meir Sagit,
Malitsky Sergey,
Isaacson Tal,
Prusky Dov,
Leshkowitz Dena,
Schreiber Lukas,
Granell Antonio R.,
Widemann Emilie,
Grausem Bernard,
Pinot Franck,
Rose Jocelyn K. C.,
Rogachev Ilana,
Rothan Christophe,
Aharoni Asaph
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12032
Subject(s) - cutin , cuticle (hair) , transcription factor , biology , epidermis (zoology) , microbiology and biotechnology , biochemistry , botany , gene , genetics , anatomy
Summary Fleshy tomato fruit typically lacks stomata; therefore, a proper cuticle is particularly vital for fruit development and interaction with the surroundings. Here, we characterized the tomato S l SHINE 3 ( SlSHN3 ) transcription factor to extend our limited knowledge regarding the regulation of cuticle formation in fleshy fruits. We created SlSHN3 overexpressing and silenced plants, and used them for detailed analysis of cuticular lipid compositions, phenotypic characterization, and the study on the mode of SlSHN3 action. Heterologous expression of SlSHN3 in A rabidopsis phenocopied overexpression of the A rabidopsis SHNs . Silencing of SlSHN3 results in profound morphological alterations of the fruit epidermis and significant reduction in cuticular lipids. We demonstrated that SlSHN3 activity is mediated by control of genes associated with cutin metabolism and epidermal cell patterning. As with SlSHN3 RNA i lines, mutation in the SlSHN3 target gene, SlCYP86A69 , resulted in severe cutin deficiency and altered fruit surface architecture. In vitro activity assays demonstrated that SlCYP86A69 possesses NADPH ‐dependent ω‐hydroxylation activity, particularly of C 18:1 fatty acid to the 18‐hydroxyoleic acid cutin monomer. This study provided insights into transcriptional mechanisms mediating fleshy fruit cuticle formation and highlighted the link between cutin metabolism and the process of fruit epidermal cell patterning.