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Down‐regulation of PvTRE1 enhances nodule biomass and bacteroid number in the common bean
Author(s) -
Barraza Aarón,
EstradaNavarrete Georgina,
RodriguezAlegria Maria Elena,
LopezMunguia Agustin,
Merino Enrique,
Quinto Carmen,
Sanchez Federico
Publication year - 2013
Publication title -
new phytologist
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.742
H-Index - 244
eISSN - 1469-8137
pISSN - 0028-646X
DOI - 10.1111/nph.12002
Subject(s) - trehalose , trehalase , biology , nitrogen fixation , legume , rhizobium , root nodule , nitrogenase , botany , symbiosis , biochemistry , gene , bacteria , genetics
Summary Legume–rhizobium interactions have been widely studied and characterized, and the disaccharide trehalose has been commonly detected during this symbiotic interaction. It has been proposed that trehalose content in nodules during this symbiotic interaction might be regulated by trehalase. In the present study, we assessed the role of trehalose accumulation by down‐regulating trehalase in the nodules of common bean plants. We performed gene expression analysis for trehalase ( Pv TRE 1 ) during nodule development. Pv TRE 1 was knocked down by RNA interference ( RNA i) in transgenic nodules of the common bean. Pv TRE 1 expression in nodulated roots is mainly restricted to nodules. Down‐regulation of Pv TRE 1 led to increased trehalose content (78%) and bacteroid number (almost one order of magnitude). In addition, nodule biomass, nitrogenase activity, and GOGAT transcript accumulation were significantly enhanced too. The trehalose accumulation, triggered by Pv TRE 1 down‐regulation, led to a positive impact on the legume–rhizobium symbiotic interaction. This could contribute to the agronomical enhancement of symbiotic nitrogen fixation.