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Chronic ingestion of deoxynivalenol‐contaminated diet dose‐dependently decreases the area of myenteric neurons and gliocytes of rats
Author(s) -
Rissato Débora Furlan,
Santi Rampazzo Ana Paula,
Borges Stephanie Carvalho,
Sousa Fernando Carlos,
Busso Cleverson,
Buttow Nilza Cristina,
Natali Maria Raquel Marçal
Publication year - 2020
Publication title -
neurogastroenterology and motility
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.489
H-Index - 105
eISSN - 1365-2982
pISSN - 1350-1925
DOI - 10.1111/nmo.13770
Subject(s) - ingestion , myenteric plexus , population , medicine , mycotoxin , ganglion , jejunum , endocrinology , cholinergic , biology , physiology , anatomy , food science , immunohistochemistry , environmental health
Background Deoxynivalenol (DON), a mycotoxin produced by Fusarium spp., is commonly found in cereals ingested by humans and animals. Its ingestion is correlated with hepatic, hematologic, renal, splenic, cardiac, gastrointestinal, and neural damages, according to dose, duration of exposure and species. In this work, the effects of the ingestion of DON‐contaminated diet at concentrations considered tolerable for human and animal intake were assessed. Methods Male Wistar rats aging 21 days were allotted to five groups that were given, for 42 days, diets contaminated with different concentrations of DON (0, 0.2, 0.75, 1.75, and 2 mg kg −1 of chow). Food ingestion, bodyweight, oxidative status and morphometric analyses of gliocytes, and neurons of jejunal myenteric ganglia were recorded. Key Results At these concentrations, there was no food rejection, decrease in bodyweight gain, changes in oxidative status, or loss of either neurons or gliocytes. However, DON decreased gliocyte area, general neuronal population, nitrergic, cholinergic and NADH‐diaphorase positive subpopulations and, as a result, ganglion area. Conclusions & Inferences It was concluded that, even in the absence of visible effect, DON exposure reduces cell body area of gliocytes and neurons of the myenteric plexus of the rat jejunum.