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microRNA‐mRNA network model in patients with achalasia
Author(s) -
Palmieri Orazio,
Mazza Tommaso,
Bassotti Gabrio,
Merla Antonio,
Tolone Salvatore,
Biagini Tommaso,
Cuttitta Antonello,
Bossa Fabrizio,
Martino Giuseppina,
Latiano Tiziana,
Corritore Giuseppe,
Gioffreda Domenica,
Palumbo Orazio,
Carella Massimo,
Panza Anna,
Andriulli Angelo,
Latiano Anna
Publication year - 2020
Publication title -
neurogastroenterology and motility
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.489
H-Index - 105
eISSN - 1365-2982
pISSN - 1350-1925
DOI - 10.1111/nmo.13764
Subject(s) - microrna , achalasia , messenger rna , biology , microarray , gene , rock2 , pathogenesis , digital polymerase chain reaction , microarray analysis techniques , gene silencing , gene expression , bioinformatics , esophagus , genetics , immunology , polymerase chain reaction , signal transduction , anatomy , rho associated protein kinase
Background Achalasia is a rare idiopathic disease with a complex etio‐pathogenesis still unknown. This study aimed to identify microRNA (miRNA)‐mRNA regulatory networks underlying achalasia. Methods The investigation was performed in tissue specimens from 11 patients and five controls using the microarray technology followed by an integrated bioinformatics analysis. Key Results One hundred and six miRNAs were significantly up‐regulated and 64 were down‐regulated in achalasia patients. The expression of the most 10 differential expressed miRNAs (miR‐122‐5p, miR‐133a‐3p, miR‐504‐5p, miR‐187‐3p, miR‐133b, miR‐200c‐3p, miR‐375, miR‐200b‐5p, miR‐200b‐3p, and miR203a) was confirmed by droplet digital PCR in an independent cohort. The interactions between the significant miRNAs and their targets uncovered 14 miRNA‐mRNA interacting pairs with experimentally predicted genes (ie, FN1 , ROCK2 , DPYSL2 ), and 35 pairs with not experimentally target genes (ie, SULF1 , MRVI1 , PRKG1 ); all genes were involved in immune cell trafficking , skeletal and muscular system development , nervous system development macro‐processes. Conclusion & Inferences The mRNA–miRNA regulatory networks described in this study provide new insights in the genetic background of the disease, suggesting further investigations in novel pathogenic mechanisms.