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Galaninergic intramural nerve and tissue reaction to antral ulcerations
Author(s) -
Zalecki M.,
Pidsudko Z.,
FrankeRadowiecka A.,
Wojtkiewicz J.,
Kaleczyc J.
Publication year - 2018
Publication title -
neurogastroenterology and motility
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.489
H-Index - 105
eISSN - 1365-2982
pISSN - 1350-1925
DOI - 10.1111/nmo.13360
Subject(s) - galanin , antrum , stomach , receptor , myenteric plexus , medicine , immunohistochemistry , neuropeptide , biology , pathology , endocrinology
Abstract Background Well‐developed galaninergic gastric intramural nerve system is known to regulate multiple stomach functions in physiological and pathological conditions. Stomach ulcer, a disorder commonly occurring in humans and animals, is accompanied by inflammatory reaction. Inflammation can cause intramural neurons to change their neurochemical profile. Galanin and its receptors are involved in inflammation of many organs, however, their direct participation in stomach reaction to ulcer is not known. Therefore, the aim of the study was to investigate adaptive changes in the chemical coding of galaninergic intramural neurons and mRNA expression encoding Gal, GalR1, GalR2, GalR3 receptors in the region of the porcine stomach directly adjacent to the ulcer location. Methods The experiment was performed on 24 pigs, divided into control and experimental groups. In 12 experimental animals, stomach antrum ulcers were experimentally induced by submucosal injection of acetic acid solution. Stomach wall directly adjacent to the ulcer was examined by: (1) double immunohistochemistry—to verify the changes in the number of galaninergic neurons (submucosal, myenteric) and fibers; (2) real‐time PCR to verify changes in mRNA expression encoding galanin, GalR1, GalR2, GalR3 receptors. Key Results In the experimental animals, the number of Gal‐immunoreactive submucosal perikarya was increased, while the number of galaninergic myenteric neurons and fibers (in all the stomach wall layers) remained unchanged. The expression of mRNA encoding all galanin receptors was increased. Conclusions & Interferences The results obtained unveiled the participation of galanin and galanin receptors in the stomach tissue response to antral ulcerations.