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Down‐regulation of fibronectin and the correlated expression of neuroligin in hirschsprung disease
Author(s) -
Zheng Y.,
Lv X.,
Wang D.,
Gao N.,
Zhang Q.,
Li A.
Publication year - 2017
Publication title -
neurogastroenterology and motility
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.489
H-Index - 105
eISSN - 1365-2982
pISSN - 1350-1925
DOI - 10.1111/nmo.13134
Subject(s) - neuroligin , western blot , pathogenesis , immunohistochemistry , fibronectin , pathology , biology , medicine , endocrinology , microbiology and biotechnology , gene , extracellular matrix , genetics , excitatory postsynaptic potential , inhibitory postsynaptic potential
Aim The goal of this study was to investigate the expression of fibronectin ( FN ) and the correlated abundance of neuroligins ( NL s) in the enteric nervous system ( ENS ) and to find a novel diagnostic marker in the serum of Hirschsprung disease ( HSCR ) patients. Methods The expression levels of FN , neuroligin‐1 and neuroligin‐2 were detected in 114 children with or without HSCR . The expression and localization of the NL s and FN were assessed morphologically by immunohistochemical staining. Western blot analysis and real‐time fluorescence quantitative PCR ( qPCR ) were performed to examine the correlated expression of the NL s and FN in aganglionic, transitional, and normal ganglionic colon tissues. An enzyme‐linked immunosorbent assay ( ELISA ) was performed to evaluate and compare serum FN levels between HSCR and non‐ HSCR and between long‐type HSCR and short‐type HSCR . Results These studies showed that both neuroligin‐1 and neuroligin‐2 were expressed at low levels in aganglionic segments and at intermediate levels in transitional segments compared to their high level of expression in normal tissue. In contrast, FN expression was negatively correlated, with expression in these three samples transitioning from highest to lowest. The serum FN level was higher in HSCR than in non‐ HSCR , but no significant difference between short‐type HSCR and long‐type HSCR was observed. Conclusion FN affects the expression of both neuroligin‐1 and neuroligin‐2 in HSCR , which may lead to the hypoplasia of ganglion cells in the ENS . This correlation may play a key role in the pathogenesis, diagnosis, or classification of HSCR .