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Role of hydrogen sulfide within the dorsal motor nucleus of the vagus in the control of gastric function in rats
Author(s) -
Sun H.Z.,
Yu K.H.,
Ai H.B.
Publication year - 2015
Publication title -
neurogastroenterology and motility
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.489
H-Index - 105
eISSN - 1365-2982
pISSN - 1350-1925
DOI - 10.1111/nmo.12530
Subject(s) - microinjection , dorsal motor nucleus , gastric acid , motility , secretion , chemistry , medicine , gastric emptying , endocrinology , vagus nerve , cystathionine beta synthase , stomach , biology , biochemistry , microbiology and biotechnology , cysteine , enzyme , stimulation
Background Hydrogen sulfide (H 2 S) is a gaseous messenger and serves as an important neuromodulator in the central nervous system. This study aimed to clarify the role of H 2 S within the dorsal motor nucleus of the vagus ( DMV ) in the control of gastric function in rats. Methods Cystathionine β ‐synthetase ( CBS ) is an important generator of endogenous H 2 S in the brain. We investigated the distribution of CBS in the DMV using immunohistochemical method, and the effects of H 2 S on gastric motility and on gastric acid secretion. Key Results CBS ‐immunoreactive ( IR ) neurons were detected in the rostral, intermediate and caudal DMV , with the highest number of CBS ‐ IR neurons in the caudal DMV , and the lowest in the intermediate DMV . We also found that microinjection of the exogenous H 2 S donor Na HS (0.04 and 0.08 mol/L; 0.1 μ L; n = 6; p < 0.05) into the DMV significantly inhibited gastric motility with a dose‐dependent trend, and promoted gastric acid secretion in Wistar rats. Microinjection of the same volume of physiological saline ( PS ; 0.1 μ L, n = 6, p > 0.05) at the same location did not noticeably change gastric motility and acid secretion. Conclusions & Inferences The data from these experiments suggest that the CBS that produces H 2 S is present in the DMV , and microinjection of Na HS into the DMV inhibited gastric motility and enhanced gastric acid secretion in rats.