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Loss of C‐genome‐specific markers during transgene introgression from Brassica napus to wild Brassica juncea
Author(s) -
Guan ZhengJun,
Shen BaoCheng,
Wei Wei,
Tang ZhiXi,
Stewart, Jr C. Neal
Publication year - 2020
Publication title -
nordic journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.333
H-Index - 33
eISSN - 1756-1051
pISSN - 0107-055X
DOI - 10.1111/njb.02794
Subject(s) - backcrossing , introgression , brassica , biology , genetics , genome , transgene , gene , genetic marker , genetic linkage , chromosome , botany
Transgene flow from engineered Brassica napus to wild weed relatives could potentially have an environmental effect. To evaluate the introgression of transgenic B. napus into wild Brassica juncea , the hybrid F 1 and backcross progenies derived from B. juncea (genome constitution AABB) and transgenic B. napus (AACC) crosses were investigated. C‐genome‐specific simple sequence repeat (SSR) markers corresponding to linkage groups N11–N19 in B. napus were screened and used to estimate the marker frequency in hybrid F 1 and backcross progenies. C‐genome‐specific markers could be stably detected in hybrid F 1 and backcross BC 1 plants, but were only rarely found in the BC 2 –BC 5 generations. For example, a specific SSR marker for linkage group N12 segregated in BC 2 generation but were completely lost in BC 3 –BC 5 , while a specific SSR marker of linkage group N15 segregated in BC 1 , BC 2 and BC 3 generations and was absent in more advanced backcrossed generations (BC 4 and BC 5 ). The results indicate that a certain gene regions in Brassica napus plants are transmitted at a relatively lower frequency to wild relatives, and more rapidly disappeared in subsequent backcross generations. We propose that a foreign gene or transgene that is integrated in the C‐chromosome of Brassica napus could reduce the risk of introgression in nature.