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Genetic polymorphism in Chondrilla (Asteraceae) in southern European Russia and the nature of Chondrilla juncea L
Author(s) -
Kashin Alexander S.,
Kritskaya Tatyana A.,
Parkhomenko Alena S.,
Schanzer Ivan A.
Publication year - 2019
Publication title -
nordic journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.333
H-Index - 33
eISSN - 1756-1051
pISSN - 0107-055X
DOI - 10.1111/njb.02420
Subject(s) - biology , apomixis , subgenus , lineage (genetic) , botany , internal transcribed spacer , ribosomal dna , evolutionary biology , ploidy , genus , phylogenetics , genetics , ribosomal rna , gene
We studied genetic diversity in 54 populations of nine sexual and apomictic species of the genus Chondrilla ( C. acantholepis , C. ambigua , C. brevirostris , C. canescens , C. graminea , C. juncea , C. laticoronata, C. latifolia and C. pauciflora ) in SE European Russia and neighboring territories of NW Kazakhstan. We analysed the trn T– trn F region of plastid DNA and the internal transcribed spacer of ribosomal DNA (ITS1–5.8S–ITS2) using statistical parsimony, maximum likelihood and neighbor net methods. Two major evolutionary lineages, roughly corresponding to the two subgenera traditionally recognized in the region, were revealed. Within the first evolutionary lineage (subgenus Brachyrhynchus ), the sexual diploid C. ambigua and its putatively hybrid apomictic derivatives C. brevirostris, C. laticoronata and C. pauciflora could be recognized. Their identity was also confirmed by analyses of ISSR markers. The second evolutionary lineage (subgenus Chondrilla ) comprises C. juncea, C. acantholepis, C. canescens, C. graminea and C. latifolia in European Russia, but analyses of morphological variability and the genealogy of plastid and nuclear markers favor their treatment as the single facultatively apomictic species C. juncea . The results demonstrate that an apomictic mode of reproduction does not necessarily result in the formation of genetically separated microspecies.