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Immunohistochemical profiles of I DH 1, MGMT and P 53: Practical significance for prognostication of patients with diffuse gliomas
Author(s) -
Ogura Ryosuke,
Tsukamoto Yoshihiro,
Natsumeda Manabu,
Isogawa Mizuho,
Aoki Hiroshi,
Kobayashi Tsutomu,
Yoshida Seiichi,
Okamoto Kouichiro,
Takahashi Hitoshi,
Fujii Yukihiko,
Kakita Akiyoshi
Publication year - 2015
Publication title -
neuropathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 61
eISSN - 1440-1789
pISSN - 0919-6544
DOI - 10.1111/neup.12196
Subject(s) - idh1 , isocitrate dehydrogenase , immunohistochemistry , glioma , anaplastic astrocytoma , oligodendroglioma , methyltransferase , astrocytoma , temozolomide , univariate analysis , medicine , pathology , cancer research , oncology , methylation , biology , mutation , multivariate analysis , gene , enzyme , biochemistry
Genetic and epigenetic status, including mutations of isocitrate dehydrogenase ( IDH ) and TP 53 and methylation of O 6 ‐methylguanine‐DNA methyltransferase ( MGMT ), are associated with the development of various types of glioma and are useful for prognostication. Here, using routinely available histology sections from 312 patients with diffuse gliomas, we performed immunohistochemistry using antibodies specific for I DH 1 mutation, MGMT methylation status, and aberrant p53 expression to evaluate the possible prognostic significance of these features. With regard to overall survival ( OS ), univariate analysis indicated that an I DH 1‐positive profile in patients with glioblastoma ( GBM ), anaplastic astrocytoma ( AA ), anaplastic oligoastrocytoma and oligodendroglioma, or a MGMT ‐negative profile in patients with GBM and AA were significantly associated with a favorable outcome. Multivariate analysis revealed that both profiles were independent factors influencing prognosis. The OS of patients with I DH 1‐positive/ MGMT ‐negative profiles was significantly longer than that of patients with negative/negative and negative/positive profiles. A p53 profile was not an independent prognostic factor. However, for GBM / AA patients with I DH 1‐negative/ MGMT ‐negative profiles, p53 overexpression was significantly associated with an unfavorable outcome. Thus, the immunohistochemical profiles of I DH 1 and MGMT are of considerable significance in gliomas, and a combination of I DH 1, MGMT and p53 profiles may be useful for prognostication of GBM / AA .