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Characteristics of the gastrointestinal microbiota in paired live kidney donors and recipients
Author(s) -
Chan Samuel,
Morrison Mark,
Hawley Carmel M.,
Campbell Scott B.,
Francis Ross S.,
Isbel Nicole M.,
Pascoe Elaine M.,
Johnson David W.
Publication year - 2021
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/nep.13853
Subject(s) - medicine , dysbiosis , kidney transplantation , species richness , kidney transplant , kidney , transplantation , metagenomics , physiology , gastroenterology , biology , ecology , disease , genetics , gene
Background There are few studies that have examined whether dysbiosis occurs in kidney donors and transplant recipients following kidney transplant surgery. Aim To ascertain whether changes occur in the gastrointestinal microbiota of the kidney donor and recipient following kidney transplantation. Methods Kidney transplant recipients and their donors were prospectively enrolled in a pilot study to collect one faecal sample prior to, and another faecal sample between four to eight weeks following surgery. Gastrointestinal microbiota richness, Shannon diversity measures and functional assessments of kidney donors and recipients were analysed via metagenomic sequencing. Results The study included 12 donors (median age 56 years, 6 females) and 12 recipients (median age 51 years, 3 females). Donor microbiota showed no significant changes in gastrointestinal microbiota richness, Shannon diversity, or functional assessments before and after nephrectomy. Recipient microbiota was altered post‐transplant, reflected in reductions of the mean (±SD) richness values (156 ± 46.5 to 116 ± 38.6, p = 0.002), and Shannon diversity (3.57 ± 0.49 to 3.14 ± 0.52, p = 0.007), and a dramatic increase in Roseburia spp. abundance post‐transplant (26‐fold increase from 0.16 ± 0.0091 to 4.6 ± 0.3; p = 0.006; FDR = 0.12). Functionally, the post‐transplant microbial community shifted towards those taxa using the glycolysis pathway (1.2‐fold increase; p = 0.02; FDR = 0.26) for energy metabolism, while those functions involved with reactive oxygen species degradation decreased (2.6‐fold; p = 0.006; FDR = 0.14). Conclusion Live donor kidney transplantation and standard care post‐transplant result in significant alterations in gut microbiota richness, diversity, composition and functional parameters in kidney transplant recipients but not in their kidney donors.