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Interleukin‐10‐1082 A/G polymorphism is associated with renal parenchymal damage in congenital anomalies of the kidney and urinary tract
Author(s) -
Miteva Lyuba D,
Kostadinova Emilya S,
Stanilova Spaska A
Publication year - 2019
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/nep.13229
Subject(s) - genotype , parenchyma , medicine , urinary system , genotyping , kidney , gastroenterology , allele , polymorphism (computer science) , pathology , genetics , gene , biology
Aim The aim of the study was to investigate whether the functional IL10‐1082A/G polymorphism exert a role in congenital anomalies of the kidney and urinary tract (CAKUT) in children. Also, the serum IL‐10 and its association with genotype and renal parenchymal damage in CAKUT were explored. Methods In the current case‐control study, 134 paediatric cases of CAKUT and 382 unrelated controls were included. The genotyping of IL10 ‐1082A/G polymorphism was performed by amplification refractory mutation system‐PCR and IL‐10 serum level was determined by ELISA. Results Although, the genotype and allelic frequencies of IL10 ‐1082 A/G polymorphism in cases and controls were similar (χ 2 = 0.459; P = 0.79 and χ 2 = 0.426; P = 0.51, respectively), significant different genotype distribution between patients with or without parenchymal damage/reduction was observed (χ 2 = 6.9; P = 0.032). The GG‐genotype was more frequent in cases with renal parenchymal damage/reduction compared to patients with preserved parenchyma (22% vs. 9%; OR = 2.987; 95% CI = 0.979–9.468; P = 0.031). On the contrary, the heterozygous genotype was less frequent among cases with parenchymal damage/reduction compared to cases with preserved parenchyma (39% vs. 59%; OR = 0.453; 95% CI = 0.214–0.958; P = 0.024). Additionally, the serum IL‐10 was significantly higher in CAKUT patients compared to age‐sex‐matched controls (median 11.98; IQR: 7.14–31.6 vs. 5.92; IQR: 4.68–14.8; P = 0.0057). Among carriers of GG‐genotype significantly higher IL‐10 level was detected in cases with parenchymal damage/reduction, than cases with preserved parenchyma ( P = 0.028). Conclusion Our results suggested that the functional ‐1082A/G polymorphism in IL10 is associated with risk of renal parenchymal damage/reduction rather than genetic predisposition to CAKUT. Additionally, our study supposes that immunoregulatory cytokine IL‐10 might have a significant role in CAKUT.