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Utility of urinary tubular markers for monitoring chronic tubulointerstitial injury after ischemia–reperfusion
Author(s) -
Ichikawa Daisuke,
KamijoIkemori Atsuko,
Sugaya Takeshi,
Ohata Keiichi,
Hisamichi Mikako,
Hoshino Seiko,
Kimura Kenjiro,
Shibagaki Yugo
Publication year - 2018
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/nep.12998
Subject(s) - medicine , lipocalin , urinary system , nephrectomy , creatinine , urology , kidney , acute kidney injury , ischemia , albumin , endocrinology , gastroenterology
Aim The aim of this study was to elucidate whether urinary tubular markers during the chronic phase of acute kidney injury (AKI) are associated with chronic tubulointerstitial damage. Methods Male human L‐type fatty acid binding protein ( L‐FABP ) chromosomal transgenic (Tg) mice underwent ischaemic reperfusion (I/R) injury via renal pedicle clamping for either 10 min or 20 min. Contralateral nephrectomy was performed at the time of tissue reperfusion. The kidneys were analyzed 20 days after the last I/R. Results Serum creatinine levels 20 days post‐I/R were significantly higher in the 20 min I/R than in the 10 min I/R and control groups and were similar between the 10 min I/R and control groups. The degree of tubulointerstitial damage 20 days post‐I/R was significantly more severe in the 20 min I/R than in the 10 min I/R and control groups, as well as in the 10 min I/R than in the control group. Urinary levels of human L‐FABP, albumin, and kidney injury molecule‐1 (KIM‐1) 20 days post‐I/R were significantly higher in the 20 min I/R than in the control group, whereas urinary L‐FABP was significantly higher in the 10 min I/R than in the control group. Conversely, urinary neutrophil gelatinase‐associated lipocalin levels did not significantly differ between the three groups. Finally, the urinary levels of human L‐FABP, albumin, and KIM‐1 levels 20 days post‐I/R were significantly correlated with the degree of renal damage. Conclusions Urinary levels of human L‐FABP, albumin and, KIM‐1 may be useful for monitoring AKI‐to‐CKD transition in clinical practice.