Premium
Expression of cleaved caspase‐3 in renal tubular cells in Plasmodium falciparum malaria patients
Author(s) -
Wichapoon Benjamas,
Punsawad Chuchard,
Viriyavejakul Parnpen
Publication year - 2017
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/nep.12715
Subject(s) - necrosis , acute tubular necrosis , medicine , acute kidney injury , apoptosis , kidney , plasmodium falciparum , creatinine , malaria , pathology , caspase 3 , programmed cell death , biology , biochemistry
Aim In Plasmodium falciparum malaria, the clinical manifestation of acute kidney injury (AKI) is commonly associated with acute tubular necrosis (ATN) in the kidney tissues. Renal tubular cells often exhibit various degrees of cloudy swelling, cell degeneration, and frank necrosis. To study individual cell death, this study evaluates the degree of renal tubular necrosis in association with apoptosis in malarial kidneys. Methods Kidney tissues from P. falciparum malaria with AKI (10 cases), and without AKI (10 cases) were evaluated for tubular pathology. Normal kidney tissues from 10 cases served as controls. Tubular necrosis was assessed quantitatively in kidney tissues infected with P. falciparum malaria, based on histopathological evaluation. In addition, the occurrence of apoptosis was investigated using cleaved caspase‐3 marker. Correlation between tubular necrosis and apoptosis was analyzed. Results Tubular necrosis was found to be highest in P. falciparum malaria patients with AKI (36.44% ± 3.21), compared to non‐AKI (15.88% ± 1.63) and control groups (2.58% ± 0.39) (all p < 0.001). In the AKI group, the distal tubules showed a significantly higher degree of tubular necrosis than the proximal tubules ( p = 0.021) and collecting tubules ( p = 0.033). Tubular necrosis was significantly correlated with the level of serum creatinine ( r = 0.596, p = 0.006), and the occurrence of apoptosis ( r = 0.681, p = 0.001). Conclusion In malarial AKI, the process of apoptosis occurs in ATN.