Premium
Micro‐vesicles derived from bone marrow stem cells protect the kidney both in vivo and in vitro by microRNA ‐dependent repairing
Author(s) -
He Juan,
Wang Yan,
Lu Xingyan,
Zhu Bei,
Pei Xiaohua,
Wu Jianqing,
Zhao Weihong
Publication year - 2015
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/nep.12490
Subject(s) - in vivo , mesenchymal stem cell , in vitro , medicine , bone marrow , transforming growth factor , microrna , andrology , microbiology and biotechnology , pathology , chemistry , endocrinology , biology , biochemistry , gene
Aims Micro‐vesicles ( MVs ) from bone mesenchymal stem cells ( MSCs ) have been shown to contribute to the recovery of damaged kidney. The aims of the present study are to investigate the biological effects and repair mechanisms of MVs . Methods Micro‐vesicles were obtained from MSC supernatants. In vitro, the proximal tubular epithelial cells ( HK ‐2) were treated with transforming growth factor ( TGF ‐β1). The expressions of E ‐cadherin and α‐smooth muscle actin (α‐ SMA) were evaluated. In vitro, the mice were divided into: control, unilateral ureteral obstruction ( UUO ), UUO + MSC , and UUO + MV group. MVs and MSCs were injected after surgery. The mice were killed 7/14 days after surgery and handled for further tests. The micro‐ RNA expressions were labeled using the miRCURY Hy3/Hy5 Power labeling kit and hybridized on the miRCURY LNA A rray. Results In vitro, MV reversed transforming growth factor‐β1 ( TGF ‐β1)‐induced morphological changes, and firmed the expression of E‐cadherin and reduced the secretion of α‐ SMA in HK2 cells. In vivo, the level of blood urea nitrogen ( BUN ) in the MV and MSC group was lower than the UUO ( P < 0.01). The Scr level decreased after 7 days of MV treatment ( P < 0.05). Administration of MSC and MV reduced S cr level at day 14 ( P < 0.05). The level of serum UA decreased with MV administration (day 7,14, P < 0.01). Herein, a total of 503 expressed miRNAs were detected, of which, 266 were in MSC , including 237 in MVs . Conclusion Micro‐vesicles ( MVs ) protect kidneys both in vivo and vitro, and MVs are superior to MSCs in some respects. MVs can be a potential therapy in treatment of kidney diseases.