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Study of micro RNA in diabetic nephropathy: Isolation, quantification and biological function
Author(s) -
Kantharidis Phillip,
Hagiwara Shinji,
Brennan Eoin,
McClelland Aaron D.
Publication year - 2015
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1111/nep.12374
Subject(s) - microrna , diabetic nephropathy , medicine , disease , extracellular matrix , nephropathy , function (biology) , rna , fibrosis , bioinformatics , cancer research , computational biology , diabetes mellitus , microbiology and biotechnology , endocrinology , biology , gene , genetics
In recent years, several studies have reported dysregulation of micro RNA expression in disease with a growing interest focussed on targeting micro RNA s as a novel therapy for human disease. This is especially true in diabetic nephropathy where the expression of several micro RNA s is dysregulated, contributing to the increased expression and accumulation of extracellular matrix proteins and increased pro‐fibrotic signalling, ultimately resulting in renal fibrosis. The development of various techniques and micro RNA reagents has enabled work to progress very rapidly in this area. In the present article, the authors describe the methods they have used that have enabled them to contribute to our current understanding of the role of micro RNA s in diabetic nephropathy.