Premium
The protein expression profile of meningioma cells is associated with distinct cytogenetic tumour subgroups
Author(s) -
Domingues Patrícia Henriques,
Teodósio Cristina,
Otero Álvaro,
Sousa Pablo,
Gonçalves Jesus Maria,
Nieto Ana Belen,
Lopes Maria Celeste,
Oliveira Catarina,
Orfao Alberto,
Tabernero Maria Dolores
Publication year - 2015
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/nan.12127
Subject(s) - meningioma , monosomy , biology , pathology , flow cytometry , fluorescence in situ hybridization , cytogenetics , immunohistochemistry , cancer research , karyotype , microbiology and biotechnology , gene , chromosome , immunology , medicine , genetics
Aims Limited information exists about the impact of cytogenetic alterations on the protein expression profiles of individual meningioma cells and their association with the clinicohistopathological characteristics of the disease. The aim of this study is to investigate the potential association between the immunophenotypic profile of single meningioma cells and the most relevant features of the tumour. Methods Multiparameter flow cytometry ( MFC ) was used to evaluate the immunophenotypic profile of tumour cells ( n = 51 patients) and the A ffymetrix U 133 A chip was applied for the analysis of the gene expression profile ( n = 40) of meningioma samples, cytogenetically characterized by interphase fluorescence in situ hybridization. Results Overall, a close association between the pattern of protein expression and the cytogenetic profile of tumour cells was found. Thus, diploid tumours displayed higher levels of expression of the CD 55 complement regulatory protein, tumours carrying isolated monosomy 22/del(22q) showed greater levels of bcl2 and PDGFRβ and meningiomas carrying complex karyotypes displayed a greater proliferation index and decreased expression of the CD 13 ectoenzyme, the CD 9 and CD 81 tetraspanins, and the Her 2/neu growth factor receptor. From the clinical point of view, higher expression of CD 53 and CD 44 was associated with a poorer outcome. Conclusions Here we show that the protein expression profile of individual meningioma cells is closely associated with tumour cytogenetics, which may reflect the involvement of different signalling pathways in the distinct cytogenetic subgroups of meningiomas, with specific immunophenotypic profiles also translating into a different tumour clinical behaviour.