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Indirekte Immunfluoreszenzfärbung und Kreuzimmunelektrophorese zur Differenzierung von Candida albicans und Geotrichum candidum
Author(s) -
Jensen H. E.,
Hau J.,
Aalbæk B.,
Schønheyder H.
Publication year - 1990
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.1990.33.11-12.519
Subject(s) - geotrichum , candida albicans , staining , immunofluorescence , immunoelectrophoresis , microbiology and biotechnology , chemistry , biology , pathology , antibody , medicine , immunology
Summary Rabbit hyperimmune antisera were used for differential diagnosis of Candida albicans and Geotrichum candidum in murine and bovine tissues by indirect immunofluorescence (IIF) staining. Although the antisera did not detect common antigens by crossed immunoelectrophoresis (XIE) a marked cross‐reactivity of fungal cells was observed in the IIF assay. Following liquid phase absorption with heterologous antigenic preparations the hyperimmune antisera were rendered specific for each species thus making possible an unequivocal differentiation of the two species.