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MALDI‐TOF MS characterisation, genetic diversity and antifungal susceptibility of Trichosporon species from Iranian clinical samples
Author(s) -
Ahangarkani Fatemeh,
Ilkit Macit,
Vaseghi Narges,
Zahedi Nina,
Zomorodian Kamiar,
Khodavaisy Sadegh,
Afsarian Mohammad Hosein,
Abbasi Kiana,
de Groot Theun,
Meis Jacques F.,
Badali Hamid
Publication year - 2021
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.13306
Subject(s) - trichosporon , voriconazole , fluconazole , microbiology and biotechnology , broth microdilution , amphotericin b , biology , posaconazole , minimum inhibitory concentration , antifungal , yeast , antibiotics , genetics
Background Trichosporonosis is an emerging fungal infection caused by Trichosporon species, a genus of yeast‐like fungi, which are frequently encountered in human infections ranging from mild cutaneous lesions to fungemia in immunocompromised patients. The incidence of trichosporonosis has increased in recent years, owing to higher numbers of individuals at risk for this infection. Although amphotericin B, posaconazole and isavuconazole are generally effective against Trichosporon species, some isolates may have variable susceptibility to these antifungals. Objectives Herein, we evaluated the species distribution, genetic diversity and antifungal susceptibility profiles of Trichosporon isolates in Iran. Methods The yeasts were identified by matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry (MALDI‐TOF MS). Phylogenetic analysis was performed based on amplified fragment length polymorphism (AFLP). The in vitro susceptibilities of eight antifungal agents were analysed using the Clinical and Laboratory Standards Institute broth microdilution methods. Results The isolates belonged to the species T asahii ( n = 20), T japonicum ( n = 4) and T faecale ( n = 3). A dendrogram of the AFLP analysis demonstrated that T asahii and non‐ asahii Trichosporon strains ( T japonicum and T faecale ) are phylogenetically distinct. While voriconazole was the most active agent (GM MIC = 0.075 μg/ml), high fluconazole MICs (8 μg/ml) were observed for a quarter of Trichosporon isolates. The GM MIC value of amphotericin B for T asahii and non‐ asahii Trichosporon species was 0.9 μg/ml. Conclusions The distribution and antifungal susceptibility patterns of the identified Trichosporon species could inform therapeutic choices for treating these emerging life‐threatening fungi.