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Specific microRNA/mRNA expression profiles and novel immune regulation mechanisms are induced in THP‐1 macrophages by in vitro exposure to Trichosporon asahii
Author(s) -
Zhang Mingwang,
Xia Zhikuan,
Yang Xin,
Ao Junhong,
Yang Rongya
Publication year - 2021
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.13268
Subject(s) - biology , kegg , microrna , immune system , in vitro , messenger rna , microbiology and biotechnology , gene , gene expression , genetics , transcriptome
Background Trichosporon asahii is considered the most prominent species associated with invasive trichosporonosis, but little is known about the pathogenesis of T. asahii infection in the host. MicroRNAs (miRNAs) are a class of noncoding endogenous small RNAs that play vital roles by manipulating immune responses against pathogenic microorganisms. Nevertheless, the exact functions of miRNAs in T. asahii infection are still unknown. Objective To investigate the interactions involved in the miRNA immune response in THP‐1 macrophages following in vitro exposure to T. asahii . Methods We utilized next‐generation sequencing to detect differentially expressed (DE) miRNAs and mRNAs in THP‐1 cells after 24 h of in vitro exposure to T. asahii . Real‐time quantitative polymerase chain reaction (RT‐qPCR) was used to verify the sequencing results. The miRNA‐mRNA regulatory network was constructed with the DE miRNAs and DE mRNAs. We performed Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis of the predicted targeting mRNAs in the miRNA‐mRNA network. A dual‐luciferase reporter assay and enzyme‐linked immunosorbent assay (ELISA) were utilized to demonstrate the reliability of the miR‐342‐3p/Dectin‐1 pair. Results A total of 120 DE miRNAs and 588 DE mRNAs were identified after 24 h of in vitro exposure to T. asahii . The miRNA‐mRNA regulatory network was constructed with 39 DE miRNAs and 228 DE mRNAs. KEGG pathway analysis revealed that the up‐regulated DE mRNAs in the complex interaction network were mainly involved in immune‐related pathways. In addition, we verified the target relationship between miR‐342‐3p and Dectin‐1 and found that miR‐342‐3p could promote the expression of TNF‐α and IL‐6 by negatively regulating Dectin‐1. Conclusions This study evaluated the expression profiles of miRNA/mRNA and revealed the immunological consequences of THP‐1 macrophages in response to T. asahii exposure. Moreover, our data suggest that miR‐342‐3p can indirectly promote inflammatory responses and may be a potential therapeutic target against trichosporonosis.