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DNA topoisomerase 2 gene polymorphism in dermatophytes
Author(s) -
Shamsizadeh Forough,
Pchelin Ivan M.,
Makimura Koichi,
Alshahni Mohamed Mahdi,
Satoh Kazuo,
Katiraee Farzad,
Ahmadi Bahram,
RezaeiMatehhkolaei Ali
Publication year - 2020
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.13086
Subject(s) - paraphyly , polyphyly , biology , genetics , dna gyrase , internal transcribed spacer , clade , genomic dna , gene , dermatophyte , dna sequencing , dna , evolutionary biology , ribosomal rna , phylogenetics , microbiology and biotechnology , escherichia coli
Summary Background Dermatophytes are a group of keratinophilic fungi of medical importance. Despite a relatively long history of molecular taxonomic studies, there is still a need for information on genetic polymorphism in wider variety of genomic loci. Objectives Our goal was to study partial DNA topoisomerase 2 gene ( TOP2 ) polymorphism in dermatophytes. Methods We performed DNA sequencing of TOP2 in 26 dermatophyte species along with ribosomal internal transcribed spacer (ITS) sequencing. Results The number of polymorphic sites in TOP2 data set was similar to that one in ITS data set. Nannizzia species formed paraphyletic group in TOP2 tree. Trichophyton simii was paraphyletic in concatenated TOP2 ‐ITS tree, one of its two clades contained solely Iranian isolates. Conclusions Our results revealed several unresolved problems in the taxonomy of dermatophytes, including probable polyphyly of the genus Nannizzia and the species T simii .