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Clinical isolates of Trichophyton rubrum are completely inhibited by photochemical treatment with a γ‐cyclodextrin formulation of curcuminoids
Author(s) -
Brasch Jochen,
BeckJendroschek Vera,
Walther Grit,
Rubbel Darian
Publication year - 2020
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.13051
Subject(s) - curcuminoid , curcumin , trichophyton rubrum , chemistry , cyclodextrin , curcuma , irradiation , nuclear chemistry , phototoxicity , microbiology and biotechnology , chromatography , biochemistry , biology , botany , in vitro , antifungal , physics , nuclear physics
Summary Introduction It was shown previously that dermatophytes can markedly be inhibited by a photochemical treatment with curcumin. This kind of photo‐inactivation needs to be improved, however, because curcumin is poorly water‐soluble. Therefore, a new water‐soluble γ‐cyclodextrin formulation of curcuminoids was tested for its photochemical inactivation of Trichophyton ( T . ) rubrum . Materials and methods Conidia were harvested from 6 typical strains of T rubrum and used to inoculate wells of microtiter plates. These wells were also filled with a γ‐cyclodextrin curcuminoid formulation with 0.1% DMSO and Sabouraud broth. The assays were then irradiated with visible light (wavelength 420 nm, 45 J/cm 2 ). After 24 hours, curcuminoid was added once more, and irradiation was repeated. Fungal growth was monitored photometrically for 8 days and compared with controls. Results Growth of all 6 T rubrum strains was completely inhibited by the photochemical treatment with the γ‐cyclodextrin formulation of curcuminoids. The same curcuminoid formulation applied without irradiation had only a minor inhibitory effect. Discussion Photo‐inactivation of dermatophytes with a γ‐cyclodextrin formulation of curcuminoids plus visible light is a very promising procedure with potential for a new treatment of patients with superficial tinea.