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Discrimination of Aspergillus flavus from Aspergillus oryzae by matrix‐assisted laser desorption/ionisation time‐of‐flight (MALDI‐TOF) mass spectrometry
Author(s) -
Hedayati Mohammad T.,
TaghizadehArmaki Mojtaba,
Zarrinfar Hossein,
Hoseinnejad Akbar,
Ansari Saham,
Abastabar Mahdi,
Er Halil,
Özhak Betil,
Öğünç Dilara,
Ilkit Macit,
Seyedmousavi Seyedmojtaba
Publication year - 2019
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.13010
Subject(s) - aspergillus flavus , aspergillus oryzae , aspergillus , microbiology and biotechnology , biology , aflatoxin , food science , fermentation
Summary Background Aspergillus flavus is a major cause of severe non‐invasive fungal infections in the Middle Eastern countries. However, it is difficult to distinguish A flavus from A oryzae . Objectives To assess the potential of matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry (MALDI‐TOF MS) in discriminating between A flavus and A oryzae and compare it with β‐tubulin gene sequencing. Methods We used the Bruker Daltonik MALDI‐TOF MS system to analyse 200 clinical and environmental A flavus isolates and one A pseudonomius and one A alliaceus ( Aspergillus section Flavi ) isolate a priori identified as such by sequencing of the β‐tubulin gene. Results All 200 A flavus isolates were identified at the genus level and 176 (88%) at the species levels by MALDI‐TOF MS based on the spectral log‐scores (≥2.0 and 1.7‐1.99, respectively); among them, only 18 (10.2%) were confirmed as A flavus , whereas 35 (19.9%) were identified as A oryzae and 123 (69.9%) as A flavus/A oryzae . Aspergillus pseudonomius and A alliaceus were misidentified as A flavus and A parasiticus with log‐score values of 1.39 and 1.09, respectively. Conclusions The results indicate that the commercially available Bruker Daltonik MALDI‐TOF MS score database cannot separate A flavus and A oryzae species. We also showed that establishment of an in‐house library is a useful tool to discriminate closely related Aspergillus species, including A flavus and A oryzae .