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Mating genotypes and susceptibility profiles of clinical isolates of Candida glabrata from Turkey
Author(s) -
Kaplan Engin,
Aktaş Deniz,
Önder Şükran,
Metin Banu,
Döğen Aylin,
Oz Yasemin,
Ilkit Macit
Publication year - 2019
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12945
Subject(s) - anidulafungin , candida glabrata , biology , caspofungin , microbiology and biotechnology , posaconazole , fluconazole , voriconazole , locus (genetics) , genotype , mating type , amphotericin b , allele , genetics , gene , candida albicans , antifungal
Summary The sexual cycle of Candida glabrata is not known; however, genomic evidence is indicative of recombination among subpopulations and the genome harbours genes necessary for undergoing mating and meiosis, which may increase fitness. The relationship between specific mating type‐like ( MTL ) loci and antifungal susceptibility is not well understood in C . glabrata . We investigated different combinations of clinical C . glabrata isolate mating types and their antifungal susceptibility profiles. Allele profiles of the mating genes of 103 clinical C . glabrata isolates were identified, and their antifungal susceptibility to azoles, echinocandins and amphotericin B were compared. The majority (88.3%) of screened isolates harboured the a allele in the locus. The MTL1 , MTL2 and MTL3 loci harboured a (88.3%), a (95.1%), and α (71.8%) alleles, respectively. The C . glabrata isolates were susceptible to echinocandins but displayed high minimal inhibitory concentrations (MICs) for azoles. The MIC ranges and MIC90 values of all isolates were 1.0 to ≥64 and 8.0 μg mL −1 for fluconazole, 0.06 to ≥16.0 and 0.5 μg mL −1 for voriconazole, 0.06 to ≥16.0 and 1.0 μg mL −1 for posaconazole, ≤0.015 to 0.06, and 0.03 μg mL −1 for caspofungin, ≤0.015 to 0.06 and 0.015 μg mL −1 for anidulafungin and 0.5‐2 and 2.0 μg mL −1 for amphotericin B, respectively. The mating gene alleles of the clinical C . glabrata isolates were not associated with differences in the MICs of the tested antifungals, except for the MTL3 α‐allele and echinocandins. The mating genotypes of the clinical C . glabrata isolates had no recognisable common effect on antifungal susceptibility.