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Comparison of genotyping methods for Cunninghamella bertholletiae
Author(s) -
Verhasselt Hedda Luise,
Radke Julia,
Schmidt Dirk,
Killengray David,
Scharmann Ulrike,
Rickerts Volker,
Hansen Wiebke,
Seidel Danila,
FalcesRomero Iker,
Buer Jan,
Rath PeterMichael,
Steinmann Joerg
Publication year - 2019
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12908
Subject(s) - genotyping , mucorales , biology , microsatellite , genotype , rapd , polymerase chain reaction , mucor circinelloides , genetics , microbiology and biotechnology , mucormycosis , genetic diversity , aspergillus , medicine , mucor , population , allele , gene , environmental health , pathology
Summary Background Invasive fungal infections caused by filamentous fungi of the order Mucorales are serious complications in immunocompromised patients and often associated with fatal outcome. As a member of this order, Cunninghamella bertholletiae is a saprophytic fungus with naturally exhibited high minimum inhibitory concentrations against common antifungal drugs and with the potential for outbreaks in clinical settings. Objectives and methods In a proof‐of‐principle study, we evaluated the performance of microsatellite markers for the discrimination of thirteen C. bertholletiae isolates from various sources in comparison with a repetitive sequence‐based PCR (rep‐ PCR ) and random amplification of polymorphic DNA ( RAPD ). Based on the higher discriminatory power of the microsatellite PCR with five separate primer pairs (Simpson's index of 1 vs 0 [ RAPD ] and 0 [rep‐ PCR ]), the novel method was applied to eight additional isolates, including four well‐characterised isolates from a cluster of infections in a next step. Results In total, microsatellite PCR identified 21 separate genotypes. A probable epidemiological association of the cluster isolates could be demonstrated by microsatellite genotyping. Conclusion In conclusion, our findings demonstrate the value of microsatellite PCR in genotyping Cunninghamella bertholletiae and its potential for future applications with other species of the order Mucorales.