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A high‐throughput and rapid method for accurate identification of emerging multidrug‐resistant Candida auris
Author(s) -
Ahmad Ausaf,
Spencer Jonathan E.,
Lockhart Shawn R.,
Singleton Sabrina,
Petway David J.,
Bagarozzi Dennis A.,
Herzegh Owen T.
Publication year - 2019
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12907
Subject(s) - candida auris , taqman , dna extraction , turnaround time , multiple drug resistance , medicine , real time polymerase chain reaction , biology , microbiology and biotechnology , polymerase chain reaction , drug resistance , antifungal , computer science , genetics , gene , operating system
Summary Candida auris is an emerging multidrug‐resistant yeast associated with invasive infection in healthcare settings. Recently, C auris cases in the United States have been detected in 11 states with the majority of cases in New York, New Jersey and Illinois. Rapid and accurate identification of C auris is critical for patient care and the implementation of public health measures to control the spread of infection. Our aim was to develop and validate a rapid DNA extraction method using the Roche Mag NA Pure 96 instrument and a TaqMan real‐time PCR assay for reliable, high‐throughput identification of C auris . We evaluated 247 patient dermal swab samples previously analysed by culture/ MALDI ‐ TOF . The diagnostic sensitivity and specificity were 93.6% and 97.2%, respectively. The assay was highly reproducible with a detection limit of 1 C auris CFU /10 μL. A receiver operating characteristic curve analysis of the real‐time PCR data showed an area of 0.982 under the curve, with a C T cut‐off value of ≤37.0. The turnaround time from DNA extraction to real‐time PCR results was approximately 200 samples/day. In conclusion, we successfully validated a rapid and high‐throughput method for accurate and reproducible identification of C auris with a significantly reduced turnaround time compared to culture/ MALDI ‐ TOF based methods.