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In vitro activity of diphenyl diselenide and ebselen alone and in combination with antifungal agents against Trichosporon asahii
Author(s) -
Felli Kubiça Thaís,
Bedin Denardi Laura,
Silva de Loreto Érico,
Zeni Gilson,
Weiblen Carla,
Oliveira Vanessa,
Morais Santurio Janio,
Hartz Alves Sydney
Publication year - 2019
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12906
Subject(s) - fluconazole , diphenyl diselenide , caspofungin , chemistry , amphotericin b , in vitro , itraconazole , azole , in vivo , microbiology and biotechnology , pharmacology , antifungal , biology , biochemistry , selenium , organic chemistry
Summary This study evaluated the in vitro susceptibility of Trichosporon asahii strains to diphenyl diselenide ( DPDS ) and ebselen ( EBS ) alone and in combination with amphotericin B ( AMB ), fluconazole ( FCZ ), itraconazole ( ITZ ) and caspofungin ( CAS ) using the microdilution method. EBS showed in vitro activity against T asahii strains with minimal inhibitory concentration ( MIC ) ranged from 0.25 to 8.0 μg/mL. For DPDS , the MIC ranged from 8.0 to 64 μg/mL. The combinations demonstrating the greatest synergism rate against fluconazole‐resistant T asahii strains were the following: CAS + DPDS (96.67%), AMB + DPDS (93.33%), FCZ + DPDS (86.67%) and ITZ + DPDS (83.33%). The combinations AMB + DPDS and AMB + EBS exhibited the highest synergism rate against the fluconazole‐susceptible ( FS ) T asahii strains (90%). Antagonism was observed in the following combinations: FCZ + EBS (80%) and FCZ + DPDS (13.33%) against the FS strains, and ITZ + EBS (20%) against the FR strains. Our findings suggest that the antimicrobial activity of DPDS and EBS against T. asahii and its use as an adjuvant therapy with antifungal agents warrant in vivo experimental investigation.