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In vitro activity of azole derivatives and griseofulvin against planktonic and biofilm growth of clinical isolates of dermatophytes
Author(s) -
Brilhante Raimunda Sâmia Nogueira,
Correia Edmilson Emanuel Monteiro,
Guedes Glaucia Morgana de Melo,
Oliveira Jonathas Sales,
CasteloBranco Débora de Souza Collares Maia,
Cordeiro Rossana de Aguiar,
Pinheiro Adriana de Queiroz,
Chaves Lúcio Jackson Queiroz,
Pereira Neto Waldemiro de Aquino,
Sidrim José Júlio Costa,
Rocha Marcos Fábio Gadelha
Publication year - 2018
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12763
Subject(s) - griseofulvin , microbiology and biotechnology , dermatophyte , microsporum gypseum , microsporum canis , itraconazole , biofilm , trichophyton rubrum , minimum inhibitory concentration , voriconazole , trichophyton , biology , chemistry , antimicrobial , medicine , bacteria , dermatology , antifungal , genetics
Summary As shown by recent research, most of the clinically relevant fungi, including dermatophytes, form biofilms in vitro and in vivo, which may exhibit antimicrobial tolerance that favour recurrent infections. The aim of this study was to determine the minimum inhibitory concentrations ( MIC s) of itraconazole ( ITC ), voriconazole ( VCZ ) and griseofulvin ( GRI ) against Trichophyton rubrum , Trichophyton tonsurans , Trichophyton mentagrophytes , Microsporum canis and Microsporum gypseum in planktonic and biofilm growth. For the planktonic form, susceptibility testing was performed according to the Clinical and Laboratory Standards Institute ( CLSI ), document M38‐A2, while biofilm susceptibility was evaluated using the XTT colorimetric essay. The planktonic growth of all strains was inhibited, with MIC values ranging from 0.00195 to 0.1225 μg/ mL for VRC , 0.00195 to 0.25 μg/ mL for ITC and <0.0039 to 4 μg/ mL for GRI , while a 50‐fold increase in the MIC was required to significantly reduce the metabolic activity ( P < .05) of dermatophyte biofilms. In brief, the ability of dermatophytes to form biofilms may be a contributing factor for the recalcitrance of dermatophytoses or the dissemination of the disease.