Evaluation of the new  Asp  ID  polymerase chain reaction assay for detection of  Aspergillus  species: A pilot study | Zendy

Prattes Juergen | Zendy; Hoenigl Martin | Zendy; Zinke Stefanie E.M. | Zendy; Heldt Sven | Zendy; Eigl Susanne | Zendy; Johnson Gemma L. | Zendy; Bustin Stephen | Zendy; Stelzl Evelyn | Zendy; Kessler Harald H. | Zendy

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Evaluation of the new Asp ID polymerase chain reaction assay for detection of Aspergillus species: A pilot study

Author(s) -

Prattes Juergen,

Hoenigl Martin,

Zinke Stefanie E.M.,

Heldt Sven,

Eigl Susanne,

Johnson Gemma L.,

Bustin Stephen,

Stelzl Evelyn,

Kessler Harald H.

Publication year - 2018

Publication title -

mycoses

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.13

H-Index - 69

eISSN - 1439-0507

pISSN - 0933-7407

DOI - 10.1111/myc.12757

Subject(s) - bronchoalveolar lavage , aspergillus , polymerase chain reaction , aspergillosis , microbiology and biotechnology , medicine , chromatography , chemistry , biology , immunology , lung , gene , biochemistry

Summary The newly developed Asp ID PCR assay for detection of Aspergillus spp. was evaluated with an interlaboratory quality control programme panel and human bronchoalveolar lavage fluid ( BALF ) samples. With the quality control programme, 8 out of 9 panel members were correctly identified. With the clinical study, 36 BALF samples that had been obtained from 18 patients with invasive pulmonary aspergillosis ( IPA ) and 18 without IPA were investigated. Sensitivity, specificity, positive and negative likelihood ratio for the Asp ID assay were 94.1% (95% CI 73.3‐99.9), 76.5% (95% CI 50.1‐93.2), 4 (95% CI 1.7‐9.5) and 0.1 (95% CI 0.01‐0.5) respectively.

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