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Evaluation of the new Asp ID polymerase chain reaction assay for detection of Aspergillus species: A pilot study
Author(s) -
Prattes Juergen,
Hoenigl Martin,
Zinke Stefanie E.M.,
Heldt Sven,
Eigl Susanne,
Johnson Gemma L.,
Bustin Stephen,
Stelzl Evelyn,
Kessler Harald H.
Publication year - 2018
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12757
Subject(s) - bronchoalveolar lavage , aspergillus , polymerase chain reaction , aspergillosis , microbiology and biotechnology , medicine , chromatography , chemistry , biology , immunology , lung , gene , biochemistry
Summary The newly developed Asp ID PCR assay for detection of Aspergillus spp. was evaluated with an interlaboratory quality control programme panel and human bronchoalveolar lavage fluid ( BALF ) samples. With the quality control programme, 8 out of 9 panel members were correctly identified. With the clinical study, 36 BALF samples that had been obtained from 18 patients with invasive pulmonary aspergillosis ( IPA ) and 18 without IPA were investigated. Sensitivity, specificity, positive and negative likelihood ratio for the Asp ID assay were 94.1% (95% CI 73.3‐99.9), 76.5% (95% CI 50.1‐93.2), 4 (95% CI 1.7‐9.5) and 0.1 (95% CI 0.01‐0.5) respectively.