Premium
Susceptibility of Malassezia pachydermatis to aminoglycosides
Author(s) -
Silva Freddy Alejandro,
CondeFelipe Magnolia,
Rosario Inmaculada,
Ferrer Otilia,
Real Fernando,
Déniz Soraya,
Acosta Félix,
Padilla Daniel,
AcostaHernández Begoña
Publication year - 2017
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12665
Subject(s) - netilmicin , etest , tobramycin , gentamicin , broth microdilution , aminoglycoside , minimum inhibitory concentration , microbiology and biotechnology , chemistry , antibiotics , biology
Summary Previous studies have evaluated the action of gentamicin against Malassezia pachydermatis . The aim of this study was to evaluate in vitro susceptibility of M. pachydermatis to the aminoglycosides— gentamicin, tobramycin, netilmicin and framycetin. The minimum inhibitory concentration ( MIC ) of gentamicin was determined following methods M27‐A3 microdilution and Etest ® . The Etest ® was used to determine the minimum inhibitory concentration ( MIC ) of the tobramycin and netilmicin. The Kirby‐Bauer test was used to determine the antibiotic susceptibility to the framycetin. The MIC 50 and MIC 90 were 8.12 μg/mL and 32.5 μg/mL by microdilution method for gentamicin. The MIC 50, determined by the Etest ® , was 8 μg/mL for gentamicin and netilmicin and 64 μg/mL for tobramycin. The MIC 90 was 16 and 32 μg/mL for gentamicin and netilmicin respectively. The MIC 90 was outside of the detectable limits for tobramycin. To framycetin, 28 strains (40%) of the 70 M. pachydermatis isolates tested showed a diameter of 22 mm, 22 strains (31.42%) showed a diameter of 20 mm, 16 strains showed a diameter of ≤ 18 mm, and only 5.71% of the isolates showed a diameter of ≥ 22 mm. This study provides evidence of high in vitro activity of the aminoglycosides—gentamicin, tobramycin, netilmicin and framycetin against M. pachydermatis . For gentamicin Etest ® showed similar values of MIC 50 y MIC 90 that the obtained by microdilution method. We considered Etest ® method could be a good method for these calculations with aminoglycosides.