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Evaluation of incubation time for dermatophytes cultures
Author(s) -
Rezusta Antonio,
Fuente Sonia,
Gilaberte Yolanda,
VidalGarcía Matxalen,
Alcalá Leticia,
LópezCalleja Ana,
Ruiz Maria Angeles,
Revillo Maria José
Publication year - 2016
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12484
Subject(s) - dermatophyte , trichophyton rubrum , incubation period , epidermophyton floccosum , incubation , trichophyton , biology , microbiology and biotechnology , microsporum , antifungal , biochemistry
Summary In general, it is recommended to incubate dermatophytes cultures for a minimum of 4 weeks. Several aspects of routine fungal cultures should be evaluated in order to implement appropriate and necessary changes. The aim of this study was to determine the optimum incubation time for routine dermatophytes cultures, analysing the time to find first fungal growth by visual observation. We recorded the time when the initial growth was detected for all dermatophyte isolates during a 4‐year period. A total of 5459 dermatophyte cultures were submitted to our laboratory. From the total cultures, only 16 (1.42%) isolates were recovered over/after 17 days of incubation and only three dermatophyte species were recovered over 17 days. Fourteen isolates belong to Trichophyton rubrum , one isolate to Trichophyton mentagrophytes complex and one isolate to Epidermophyton floccosum . We concluded that an incubation period of 17 days is enough to establish a microbiological diagnosis of dermatophytosis.