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Echinocandin resistance and population structure of invasive Candida glabrata isolates from two university hospitals in Germany and Austria
Author(s) -
Klotz Ulrike,
Schmidt Dirk,
Willinger Birgit,
Steinmann Eike,
Buer Jan,
Rath PeterMichael,
Steinmann Joerg
Publication year - 2016
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12472
Subject(s) - candida glabrata , echinocandin , genotype , biology , microsatellite , genotyping , broth microdilution , population , microbiology and biotechnology , genetics , antifungal , medicine , allele , gene , fluconazole , minimum inhibitory concentration , antibiotics , environmental health
Summary Echinocandin resistance in Candida glabrata is emerging and is associated with the presence of FKS mutations. In this study, we analysed the antifungal susceptibility, presence of FKS mutations and clonality of C. glabrata blood culture isolates from two hospitals in Germany and Austria. Susceptibility testing of 64 C. glabrata bloodstream isolates from two university hospitals was performed with broth microdilution method according to EUCAST . In addition, all isolates were screened for FKS mutations. Molecular fingerprinting was performed by microsatellite PCR with three separate primer pairs and semiautomated repetitive sequenced‐based PCR (rep‐ PCR ). One C. glabrata isolate from Germany (1.5%) was echinocandin resistant, with a corresponding mutation in FKS 2 gene hot spot 1. The discriminatory power of microsatellite PCR was higher than that of rep‐ PCR (Simpson Index of 0.94 vs. 0.88); microsatellite PCR created 31 separate genotypes, whereas rep‐ PCR created 17. Predominant genotypes or clusters of isolates from Germany and Austria were present, with no epidemiological evidence of nosocomial transmissions. Although we found a low incidence of echinocandin resistance in C. glabrata in our settings, further surveillance projects in central Europe are warranted for monitoring future epidemiological trends. The genetic population structure of C. glabrata demonstrates overrepresented geographical clusters.