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Isolation and identification of Malassezia species from Chinese and Korean patients with seborrheic dermatitis and in vitro studies on their bioactivity on sebaceous lipids and IL ‐8 production
Author(s) -
Kim Soo Young,
Kim Se Hyun,
Kim Su Na,
Kim AhReum,
Kim Yu Ri,
Kim Min Jung,
Park WonSeok,
Lee John Hwan,
Jung Won Hee,
Lee Yang Won,
Choe Yong Beom,
Ahn Kyu Joong
Publication year - 2016
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12456
Subject(s) - malassezia , seborrheic dermatitis , hacat , dandruff , ceramide , scalp , biology , dermatology , in vitro , chemistry , microbiology and biotechnology , medicine , shampoo , apoptosis , biochemistry , pathology
Summary We investigated the distribution of Malassezia yeast in 120 Chinese (20 patients from each of six cities) and 20 Korean patients with scalp seborrheic dermatitis ( SD ) and dandruff ( SD /D) using ITS 1 and ITS 2 polymerase chain reaction‐restriction fragment length polymorphism. Bioactivity was studied by quantifying sebum lipid production by human primary sebocytes and inflammatory cytokine, interleukin‐8 ( IL ‐8) production was studied by exposing HaCaT keratinocytes with extracts of five standard Malassezia strains; M. globosa , M. restricta , M. sympodialis , M. dermatis and M. slooffiae. M. restricta and M. globosa were the most frequently encountered species from both Chinese and Korean patients. These two Malassezia species also promoted neutral lipid synthesis although the result was not statistically significant and induced significant increase in IL ‐8 production among the five Malassezia species studied. The study suggests a possible role of these organisms in the pathogenesis of SD /D.