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Differential effects of antifungal agents on expression of genes related to formation of Candida albicans biofilms
Author(s) -
Chatzimoschou Athanasios,
Simitsopoulou Maria,
Antachopoulos Charalampos,
Walsh Thomas J.,
Roilides Emmanuel
Publication year - 2016
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12436
Subject(s) - anidulafungin , biofilm , voriconazole , microbiology and biotechnology , candida albicans , corpus albicans , biology , gene , gene expression , micafungin , chemistry , antifungal , bacteria , biochemistry , genetics
Summary The purpose of this study was to analyse specific molecular mechanisms involved in the intrinsic resistance of C. albicans biofilms to antifungals. We investigated the transcriptional profile of three genes ( BGL 2, SUN 41, ECE 1 ) involved in Candida cell wall formation in response to voriconazole or anidulafungin after the production of intermediate and mature biofilms. C. albicans M61, a well‐documented biofilm producer strain, was used for the development of intermediate (12 h and 18 h) and completely mature biofilms (48 h). After exposure of cells from each biofilm growth mode to voriconazole (128 and 512 mg l −1 ) or anidulafungin (0.25 and 1 mg l −1 ) for 12–24 h, total RNA samples extracted from biofilm cells were analysed by RT ‐ PCR . The voriconazole and anidulafungin biofilm MIC was 512 and 0.5 mg l −1 respectively. Anidulafungin caused significant up‐regulation of SUN 41 (3.7–9.3‐fold) and BGL 2 (2.2–2.8 fold) in intermediately mature biofilms; whereas, voriconazole increased gene expression in completely mature biofilms ( SUN 41 2.3‐fold, BGL 2 2.1‐fold). Gene expression was primarily down‐regulated by voriconazole in intermediately, but not completely mature biofilms. Both antifungals caused down‐regulation of ECE 1 in intermediately mature biofilms.