Premium
Identification of invasive fungal diseases in immunocompromised patients by combining an Aspergillus specific PCR with a multifungal DNA ‐microarray from primary clinical samples
Author(s) -
Boch T.,
Reinwald M.,
Postina P.,
Cornely O. A.,
Vehreschild J. J.,
Heußel C. P.,
Heinz W. J.,
Hoenigl M.,
Eigl S.,
Lehrnbecher T.,
Hahn J.,
Claus B.,
Lauten M.,
Egerer G.,
Müller M. C.,
Will S.,
Merker N.,
Hofmann W.K.,
Buchheidt D.,
Spiess B.
Publication year - 2015
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12424
Subject(s) - aspergillus , identification (biology) , microarray , microbiology and biotechnology , dna microarray , aspergillosis , biology , polymerase chain reaction , dna , medicine , immunology , gene , genetics , gene expression , botany
Summary The increasing incidence of invasive fungal diseases ( IFD ), most of all invasive aspergillosis ( IA ) in immunocompromised patients emphasises the need to improve the diagnostic tools for detection of fungal pathogens. We investigated the diagnostic performance of a multifungal DNA ‐microarray detecting 15 different fungi [ Aspergillus, Candida, Fusarium, Mucor, Rhizopus, Scedosporium and Trichosporon species (spp.)] in addition to an Aspergillus specific polymerase chain reaction ( PCR ) assay. Biopsies, bronchoalveolar lavage and peripheral blood samples of 133 immunocompromised patients (pts) were investigated by a multifungal DNA ‐microarray as well as a nested Aspergillus specific PCR assay. Patients had proven ( n = 18), probable ( n = 29), possible ( n = 48) and no IFD ( n = 38) and were mostly under antifungal therapy at the time of sampling. The results were compared to culture, histopathology, imaging and serology, respectively. For the non‐ Aspergillus IFD the microarray analysis yielded in all samples a sensitivity of 64% and a specificity of 80%. Best results for the detection of all IFD were achieved by combining DNA ‐microarray and Aspergillus specific PCR in biopsy samples (sensitivity 79%; specificity 71%). The molecular assays in combination identify genomic DNA of fungal pathogens and may improve identification of causative pathogens of IFD and help overcoming the diagnostic uncertainty of culture and/or histopathology findings, even during antifungal therapy.