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Nutritional status and immune response in murine experimental Jorge Lobo's disease
Author(s) -
Barbosa Adriana Sierra Assencio Almeida,
Diório Suzana Madeira,
Pedrini Silvia Cristina Barboza,
Silva Sônia Maria Uso Ruiz,
Sartori Beatriz Gomes Carreira,
Calvi Sueli Aparecida,
Pereira Paulo Câmara Marques,
VilaniMoreno Fátima Regina
Publication year - 2015
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12351
Subject(s) - inoculation , immune system , malnutrition , immunity , immunology , medicine , biology
Summary There are no studies investigating the role of nutritional status and immunity associated with Jorge Lobo's disease. The objective of this study was to evaluate the effects of protein‐calorie malnutrition on the immune response of BALB/c mice inoculated with Lacazia loboi . In this study,the animals were divided into four groups: G1: inoculated with restricted diet, G2: not inoculated with restricted diet, G3: inoculated with regular diet, G4: not inoculated with regular diet. The animals of groups G1 and G2 were submitted to malnutrition for 20 days and once installed the animals were inoculated intradermally into the footpad. After 4 months, they were euthanised for the isolation of peritoneal lavage cells and removal of the footpad. The production of IL‐2, IL‐4, IL‐10, IL‐12, IFN‐γ, TNF‐α, H 2 O 2 and nitric oxide (NO) was evaluated in the peritoneal lavage cells. The footpad was evaluated regarding the size of macroscopic lesions, number of fungi and viability index. The results showed that the infection did not exert great influence on the body weight of the mice and previous malnutrition was an unfavourable factor for viability index, number of fungi, macroscopic lesion size in the footpad and production of H 2 O 2 , NO, IL‐12, IL‐10 and IFN‐γ, suggesting that malnutrition significantly altered fungal activity and peritoneal cells. The results suggest considerable interaction between nutrition and immunity in Jorge Lobo's disease.