In vitro  susceptibility testing of amphotericin B for  Cryptococcus neoformans  variety  grubii   AFLP 1/ VN I and  Cryptococcus gattii   AFLP 6/ VG II by  CLSI  and flow cytometry | Zendy

Morales Bernardina Penarrieta | Zendy; Trilles Luciana | Zendy; Bertho Álvaro Luiz | Zendy; Junior Ivan Neves | Zendy; Oliveira Raquel de Vasconcellos Carvalhaes | Zendy; Wanke Bodo | Zendy; Lazéra Márcia dos Santos | Zendy

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In vitro susceptibility testing of amphotericin B for Cryptococcus neoformans variety grubii AFLP 1/ VN I and Cryptococcus gattii AFLP 6/ VG II by CLSI and flow cytometry

Author(s) -

Morales Bernardina Penarrieta,

Trilles Luciana,

Bertho Álvaro Luiz,

Junior Ivan Neves,

Oliveira Raquel de Vasconcellos Carvalhaes,

Wanke Bodo,

Lazéra Márcia dos Santos

Publication year - 2015

Publication title -

mycoses

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.13

H-Index - 69

eISSN - 1439-0507

pISSN - 0933-7407

DOI - 10.1111/myc.12310

Subject(s) - cryptococcus gattii , cryptococcus neoformans , amplified fragment length polymorphism , cryptococcus , biology , cryptococcosis , microbiology and biotechnology , amphotericin b , broth microdilution , minimum inhibitory concentration , antifungal , antibiotics , genetic diversity , medicine , population , environmental health

Summary Cryptococcus neoformans var. grubii AFLP 1/ VNI is the main causative agent of cryptococcosis associated with AIDS in the world. Cryptococcus gattii AFLP 6/ VGII causes mainly endemic primary infection in immunocompetent hosts. To determine the minimum inhibitory concentrations ( MIC s) of C. neoformans var. grubii AFLP 1/ VNI and C. gattii AFLP 6/ VGII against amphotericin B ( AMB ) in a short period of time, flow cytometry ( FCM ) with FUN ‐1 fluorochrome was used to compare with broth microdilution method ( CLSI M27‐A3). The minimum incubation period was evaluated by minimum fungicidal concentration procedure. Seventeen clinical isolates of C. neoformans var. grubii AFLP 1/ VNI and 18 of C. gattii AFLP 6/ VGII were analysed. The time for the determination of MIC s by FCM was 2 h against 72 h by CLSI M27‐A3 and the comparison of MIC showed a positive significant correlation ( P = 0.048). It is important to highlight the role of the FCM as an alternative method to determine the MIC s for AMB in within a day, with positive cost‐benefit.

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