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Phenotypic and molecular identification of Fonsecaea pedrosoi strains isolated from chromoblastomycosis patients in Mexico and Venezuela
Author(s) -
Carolina Rojas O.,
LeónCachón Rafael B. R.,
PérezMaya Antonio Alí,
AguirreGarza Marcelino,
MorenoTreviño María G.,
González Gloria M.
Publication year - 2015
Publication title -
mycoses
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.13
H-Index - 69
eISSN - 1439-0507
pISSN - 0933-7407
DOI - 10.1111/myc.12308
Subject(s) - chromoblastomycosis , biology , posaconazole , internal transcribed spacer , microbiology and biotechnology , itraconazole , terbinafine , ribosomal dna , intergenic region , phenotype , ribosomal rna , phylogenetics , genetics , gene , antifungal , genome
Summary Chromoblastomycosis is a chronic granulomatous disease caused frequently by fungi of the Fonsecaea genus. The objective of this study was the phenotypic and molecular identification of F. pedrosoi strains isolated from chromoblastomycosis patients in Mexico and Venezuela. Ten strains were included in this study. For phenotypic identification, we used macroscopic and microscopic morphologies, carbohydrate assimilation test, urea hydrolysis, cixcloheximide tolerance, proteolitic activity and the thermotolerance test. The antifungal activity of five drugs was evaluated against the isolates. Molecular identification was performed by sequencing the internal transcribed spacer ( ITS ) ribosomal DNA regions of the isolated strains. The physiological analysis and morphological features were variable and the precise identification was not possible. All isolates were susceptible to itraconazole, terbinafine, voriconazole and posaconazole. Amphotericin B was the least effective drug. The alignment of the 559‐nucleotide ITS sequences from our strains compared with sequences of GenBank revealed high homology with F. pedrosoi ( EU285266.1 ). In this study, all patients were from rural areas, six from Mexico and four from Venezuela. Ten isolates were identified by phenotypic and molecular analysis, using ITS sequence and demonstrated that nine isolates from Mexico and Venezuela were 100% homologous and one isolate showed a small genetic distance.